/ · 1894 Brewer’s · I · Iota
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or Jot: A very little, the least quantity possible. The iöta [ι] is the smallest letter of the Greek alphabet, called the Lacedemonian letter. (Hebrew, Yod [י] the smallest Hebrew letter.)
“This bond doth give thee here no jot of blood.”
Shakespeare: Merchant of Venice, iv. 1.
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Entry taken from Dictionary of Phrase and Fable, edited by the Rev. E. Cobham Brewer, LL.D. and revised in 1895.